Williamsburg is an unincorporated community in eastern Callaway County, Missouri, USA. It is part of the Jefferson City, Missouri Metropolitan Statistical Area. It is home to Nine Mile Presbyterian church, presided over by James W. Cruickshank. It is also located on Missouri Supplemental Route D just off Interstate 70 about thirteen miles northeast of Fulton at 38°52′11″N 91°44′49″W / 38.86972°N 91.74694°W / 38.86972; -91.74694 (38.8697, -91.7469)